수간다코킬라유와 그 주성분인 시네올의 인간각화세포 및 생체피부모방모델에서

Abstract

학위논문 (석사)-- 서울대학교 대학원 : 농생명공학부, 2016. 8. 이기원.Sugnadha kokila oil (Cinnamomum glaucescens, SKO) has been widely used in folk medicine to alleviate pain and inflammation in Nepal. It has been reported that SKO has insecticidal, antifungal, antiaflatoxin and antioxidant activities. Also, 1,8-cineole, the major compound of SKO, is known for many advantage such as antimicrobial properties. It constantly consumed as a penetration enhancer for topical delivery to the skin. But the anti-aging effect of SKO and 1,8-cineole have not been studied. For these purpose, I investigated an effect of SKO and its major compound 1,8-cineole on solar UV-induced skin aging in immortalized human keratinocyte. SKO and 1,8-cineole suppressed Solar UV-induced both MMP-1 protein expression and MMP-1 gene transcription. Furthermore, 1,8-cineole suppressed AP-1 which has important role in regulating MMP expression. Also, 1,8-cineole inhibited solar UV-induced phosphorylation of MEK1/2, ERK, and p90RSK. . In addition, 1,8-cineole inhibits solar UV-induced collagenase expression and collagen degradation in 3D human skin equivalent. These result suggest that SKO and its major compound 1,8-cineole could be a potential anti-wrinkle agent for skin photoaging.Ⅰ. INTRODUCTION 1 Ⅱ. MATERIALS AND METHODS 5 1. Chemicals and reagents 5 2. Cell Culture and treatments 6 3. Gas chromatography-mass spectrometry (GCMS) 7 4. Cell viability 8 5. Preparation of human skin equivalent 9 6. Massons trichrome staining and immunohistochemistry 10 7. Western blot and zymography 12 8. Real-time quantitative PCR 14 9. Luciferase reporter gene assay 15 10. Statistical analysis 17 Ⅲ. RESULTS 18 1. Sugandha kokila oil had inhibitory effect on solar UV-induced MMP-1 protein expression and gene transcription in human keratinocyte 18 2. 1,8-cineole was a major component of SKO 22 3. 1,8-cineole effectively attenuated solar-UV induced MMP-1 protein expression and gene transcription in HaCaT cells 25 4. 1,8-cineole significantly decreased solar UV-induced AP-1 transactivation and influenced solar-induced cellular signal transduction in HaCaT cells 29 5. 1,8-cineole inhibited solar UV-induced collagenase expression and collagen degradation in 3D human skin equivalent 33 Ⅳ. DISCUSSION 36 Ⅴ. REFERENCES 40 Ⅵ. 국문 초록 44Maste