Studies of Pregnancy Test using the Male Rana Nigromaculata Hallowell., Indigenous Frog of Korea.

Abstract

A positive response was the presence of sperm in the frog s cloacal urine two hours after injecting a specimen into its dorsal lymph sac. The test was performed using a urine specimen from 120 normal pregnant women, and from 52 pathological pregnancy cases such as hydatidiform mole, chorio· epithelioma, ectopic pregnancy, abortion, missed aboI" tion and fetal death in utero. Serum specimens from 110 normal pregnant women were also studied. To shorten the response time , hyaluronidase was added to the urine and serum specimens. The test was also carried out to determine the response in either summer or winter under various temperature condit- Ions. Specimens stored at room temperature or refrigerated were tested. Responses to specimens obtained in early gestation and in the puerperal period were noted. Other body fluids such as saliva , milk , amniotic fluid and spinal fluid were also utilized to determine their effects on the frog. Response to human placental ext· ract and urine from patients with various neoplasms in addition to various hormones such as estrogen, progesterone, testosterone , cortisone, pituitrin, and adrenaline were also studied. Finally, the time·dose relationship and the sperm number-dose relationship were observed