Immunohistochemical methods, employing a specific antiserum against
human corticotropin releasing factor (CRF) and serotonin, were applied to determine
the distribution of corticotropin releasing factor-immunoreactivity (CRF-IR) and serotonin-
IR in the deep cerebellar nuclei of the Squirrel monkey. CRF-IR labeled fibers were
demonstrated in three all deep cerebellar nuclei, including the dentate, interposed and
fastigial nucleus. All these fibers were varicose and greater density was observed in
the dentate and the interposed nuclei. The serotonin-IR fibers were observed in all deep
cerebellar nuclei. But the distribution of these fibers was denser than that of CRF-IR
fibers. Labeled fibers were varicose, and there was no evidence of greater density within
deep cerebellar nuclei