Intracellular fractionation was carried out by ultracentrifugal
analysis in order to obtain the nuclear,
mitochondrial, and supernatant fractions in the liver
of mouse. Colorimetric analysis was performed to
estimate the distribution patterns of GOT and GPT
levels among the fractions.
Cortisone was administered intraperitoneally to
study the response of the transaminase activities
among the three intracellular fractions.
Along with study on the influence of cortisone, the
effects of dietary protein and fasting were investigated
to observe their effects of gluconeogenic activity
exerted upon the transaminase activity in the liver
of mouse, with the following conclusions.
1. The distributions of GOT and GPT are common
to all fractions, the activities in mitochondial fractions
being the highest and the nuclear the lowest.
2. GOT level is higher than GPT in every intra-cellular
fractions.
:l. Cortisone administration causes an increase in the activities of both transaminases in all intracellular
fractions, and its magnitude exerted upon GPT being
more stronger than GOT.
4. Conditions known to display gluconeogenic action,
such as high protein intake and fasting, cause increased
levels of GOT and GPT in all intracellular
fractions, the magnitude of which is far higher in
GPT than in GOT level, as observed with cortisone
administration.
5. In the conditions mentioned above, as cortisone
administration, increased level of mitochondrial GPT
is most pronounced among the three intracellular
fractions.
A brief discussion was made on the results, especially
on the significance of nuclear GOT and GPT as
well as variations of these enzymes in the intracellular
fractions resulting from gluconeoengesis