Follicular fluids and their matched oocytes were obtained from 16 follicles of 7
women who conceived after in vitro fertilization(IVF), and 49 follicles of 17 women who failed
to conceive after the procedure. Follicular development was induced with a combination of
follicle-stimulating hormone and human menopausal gonadotropin.
There was no significant difference in follicular cyclic AMP concentrations between preovulatory
and immature oocytes. However, follicles of preovulatory oocytes obtained from women
who conceived after IVF contained lower cyclic AMP concentrations than those from women
who failed to conceive after the procedure. Follicles of cleaved oocytes from pregnant women
contained lower cyclic AMP concentrations than those of cleaved oocytes from nonpregnant
women or all uncleaved oocytes.
These results suggest that lower cyclic AMP concentrations in follicular fluids are associated
with successful pregnancies in IVF cycles
