Etablierung eines Tiermodells zur perioperativen pharmakologischen Konditionierung von Ischämie- und Reperfusionsschäden des Rückenmarks nach Crossclamping der infrarenalen Aorta beim Kaninchen

Abstract

Paraplegia is a relevant risk after aortic crossclamping procedures. In addition to operative strategies, pharmacological conditioning may contribute to decrease spinal cord vulnerability during hypoxic conditions. Previous studies showed that erythropoietin has neuroprotective effects and molecule modification (carbamylation) reduces hematologic side effects. In large animal models the neurological outcome could not be determined due to necessity of animal sacrifice. This study established an ischemia and reperfusion injury model with new Zealand white rabbits. Functional neurologic outcome could be assessed using a modified Tarlov score in addition to histological examination. Carbamylated and native erythropoietin were tested against a placebo group for pharmacologic conditioning. Conditioning with native erythropoietin resulted in a significantly improved clinical and histological outcome compared to the carbamylated erythropoietin and placebo group. This model allowed important clinical assessment after aortic crossclamping and results were highly reproducible