It is widely recognized that the full potential of rapid chromatography can be realized with very small support particulates and tailor-made chiral stationary phase. However, there has been limited open publication in this area. This work reports the use of a novel monodispersed porous nanosized silica column in UPLC application. In this work, we have demonstrated about 3 times enhanced separation using a C18 functionalized nanosilica column compared to a commercial column. The main objectives of the project are: (1) to synthesize and functionalize silica nanoparticles and (2) apply the nanosilica for achiral drug separation. A modified Stober method is used to synthesize the silica nanoparticle. The nanoparticles were then functionalized with octadecylsilane (C18). Achiral compounds were then separated using a Ultra Performance Liquid Chromatography (UPLC).RG 37/0
