Chen, Bei

Chen, Fangyi

Dong, Lixia

Fan, Danqing

Ma, Xiaowan

Peng, Hui

Shan, Zhongguo

Wang, Kejian

Wang, Shuping

English

University of Maine

Several newly identified immune-associated components in mud crab (Scylla paramamosain) and their potential anti-infection functions   Kejian Wang1, 2, 3§, Fangyi Chen1, 2, 3, Xiaowan Ma1, Shuping Wang1, Bei Chen1, Lixia Dong1, Zhongguo Shan1, Danqing Fan1, Hui Peng1, 2, 3   1 State Key Laboratory of Marine Environmental Science, College of Ocean & Earth Science, Xiamen University, Xiamen, Fujian, P. R. China, 361005 2 Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen University, Xiamen, Fujian, P. R. China, 361005 3 Fujian Engineering Laboratory of Marine Bioproducts and Technology, Xiamen University, Xiamen, Fujian, P. R. China, 361005 This work was supported by a Grant (U1205123, 41176116) from the National Natural Science Foundation of China (NSFC).   The mud crab, Scylla paramamosain is one of the most important marine breeding crabs in China and often suffers from pathogen infection with high mortality; however few effective immune methods could be utilized for controlling or minimizing the mortality of crabs in the farms. This paper summarized a series of studies undertaken in our lab on several newly identified immune-associated components which are likely to play a very important role in protecting the crabs from infection. Transcriptomic profiling first reveals the innate immune response patterns and potential pathways associated with antimicrobial activity, proPO cascade and lectin system in the early development stages of mud crab. Several new immune-associated components including a membrane lipid rafts related gene SpFLT-1, a hemichannel-associated transmembrane protein named as Sp-inx2, two antimicrobial peptides named as Sphistin and SpHyastatin, were separately identified in different tissues of S. paramamosain. Their complete cDNA sequences and gene organization were determined, their tissue-specific distribution and expression patterns during the bacterial or fungi challenge were also evaluated. Furthermore, functional studies of these  components were performed in vivo and in vitro. The intense studies have provided new knowledge on clarifying the pathogenesis of pathogens infecting S. paramamosain, information on the key roles in immunity and cell apoptosis in the marine mud crabs and the knowledge on how these new peptides play a role against pathogenic invasion and their antimicrobial features on different species of pathogens in vitro.   Key words: Scylla paramamosain; SpFLT-1; Sp-inx2; AMPs; Anti-function;    § Corresponding author. Tel.: +86 05922184658; Fax: +86 05922184658.  E-mail address: wkjian@xmuedu.cn  

Several newly identified immune-associated components in mud crab (Scylla paramamosain) and their potential anti-infection functions

https://digitalcommons.library.umaine.edu/cgi/viewcontent.cgi?article=1168&amp;context=isfsi

Several newly identified immune-associated components in mud crab (Scylla paramamosain) and their potential anti-infection functions

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University of Maine