Imaging of biological samples in the water window offer high chemical contrast between Oxygen and Carbon. To avoid resolution limitations due to lens based microscopy techniques the coherent diffractive imaging (CDI) method is favourable for high resolution imaging. In order to measure extended samples the ptychographic coherent diffractive imaging technique (PCDI) is applied. Images of a test pattern and a fossil diatom are reconstructed with the extended ptychographic iterative engine (ePIE) at 55 nm and 286 nm resolution respectively