Leukemia is hematology malignancy caused by excess proliferation of hematopoetic or lymphoid cells. Leukemia cases in Indonesia were about 3,7 per 100.000 with mortality rate 83,6% and diagnosed based on FAB classification. The fact, WHO classification 2000, used worldwide based on cytogenesis and molecular biology profile, can define the clonal diseases more precisely and to choose the adequate therapy. CML case is about 15% of all adult leukemia cases and most of the cases are related with t(92) (q11q23) that result bcr-abl fusion gene.
The aim of this report is to show the rare case of CML that has been examined for bcr-abl fusion gene. Blood sample was obtained from CML patients diagnosed by hospital doctors based on FAB classification. Mononuclear cell was separated by Ficoll-hypaque gradient centrifugation, then RNA was isolated by Trizol and converted to cDNA by RT reaction. Beta-actin gene was used as internal control and bcr-abl gene was amplified by nested PCR.
We reported CML cases classified as atypical (case 1) and typical (case2) type based on FAB classification with post therapy for the second CML. At molecular level, bcr-abl fusion gene found at the second case with longer product than positive control.
Keywords: Leukimia,CML, fusion gene ber-ab
