Biological activity of <em>Nothoscordum bivalve </em>(L.) Britton and <em>Parthenium incanum </em>Kunth extracts

Abstract

699-706Medicinal properties of Nothoscordum bivalve (false garlic) have not been reported, while Parthenium incanum (mariola) is used to treat stomach and liver diseases. The aim of this work was to evaluate in vitro different biological activities of these plants to assess their pharmacological potential. Extracts were obtained by maceration or Soxhlet sequential solvent extraction. The extracts were tested by inhibiting the growth of Acinetobacter baumannii, besides for the techniques of Artemiasalina toxicity, cytotoxicity in human erythrocytes, cytoprotective, antioxidant capacity, quantification of soluble phenols and coagulation tests. MBC´s obtained were between 7.50±0.54 and 8.50±0.54 mg/mL. Toxicity assays showed LD50 from 380 to 1882 µg/mL. Cytotoxicity was between 0 and 92.98 %. Cytoprotective effect indicated 0 to 94.58 %. Antioxidant capacity levels of extracts were between 275.55±21 and 598.99±4 µmol TE/g. Soluble phenolics concentrations between 1.35±0.07 and 4.13±0.04 g GAE/100 g were observed. Regarding coagulation assays, only one P. incanum extract showed significant difference (p < 0.05) in the PT assay. Extracts from the sequential extraction showed less toxicity and cytotoxicity, and greater antioxidant and cytoprotective capacity; they also showed no significant prolongation in the extrinsic pathway of coagulation, and a significant (although small) prolongation in the intrinsic coagulation pathway