Micropropagation of <i style="mso-bidi-font-style: normal">Stemona tuberosa</i> Lour.—An endangered and rare medicinal plant in Eastern Ghats of India

Abstract

420-424An in vitro technique of multiple plantlet regeneration was developed for conservation of endangered wild medicinal plant Stemona tuberosa Lour.. Murashige and Skoog (MS) medium supplemented with 7.0 mg/L Kn (kinetin) was found to be the optimum for axillary bud proliferation and multiple shoot induction. Excision and culture of nodal segments from the in vitro-shoots on medium containing 7.0 mg/L Kn and 4.0 mg/L TDZ (thidiazuron) showed maximum number of shoot multiplication with 7.10±0.37 shoots/node. Rapid shoot growth with simultaneous tuberous root formation was also observed in the same concentration. Shoots developed were rooted best on ½ strength MS with 1.0 mg/L IAA (indole acetic acid). Plantlets established in pots exhibited 85% survival. Plantlets successfully established in field exhibited morphological characters identical to mother plants. </span