329-339A new organic solvent-tolerant bacterial strain UD-5 was
isolated from industrial solvent effluent-sediment stream, collected from
Ankleshwar (India)
and documented for the production of solvent-tolerant alkaline protease. Based
on phylogenetic analysis of the 16S rRNA gene sequence and phenotypic analysis,
as well as biochemical analyses, this strain was identified as Pseudomonas aeruginosa MTCC 7926. It secreted
an extracellular, thermostable; alkaline protease in a casamino acid-rich salt
medium (pH 9) at 40°C, in static condition. The solvent-tolerance of bacterium in the
presence of
n-hexane, dodecane, cyclohexane and
DMSO, xylene and tolune was analyzed. Effect of pH, temperature, agitation,
carbon and nitrogen sources and various salts on the growth and protease
production by strain UD-5 of solvent-tolerant
P. aeruginosa MTCC 7926 from-manmade ecological habitat was studied. It provided
an alternative source of alkaline protease with additional attributes of
industrial applications compared to proteases reported earlier. Its ability to grow
in organic solvents especially in water-miscible solvents (DMF and DMSO) proves
its potential for applications in
non-aqueous reactions in addition to usage in detergent, laundry additives and
leather finishing