ABSTRACT
Calanone (coumarin derivate compound), isolated from Calophyl/um sp. had been shown to have cytotoxic
activityon leukemia L1210 eel/line with IC50 =59.40 pg/mL. Calanone presumed have anticancer activity on HeLa cervical carcinoma cel/. This study was conducted to investigate the cytotoxic and apoptotic activity of calanone and its effect to p53 and p21 expression on HeLa cervical carcinqma cel/. Cytotoxic assay of calanone was performed on HeLa eel/line, using MTT assay. Apoptotic assay was performed on HeLa eel/line incubated with calanone for 24 h,
by immunofluororescence method, using fIuorochromes ethidium bromide and acridine orange. Expression of p53
was examined on HeLa eel/line, by PCR with p53 wild-type primer. Expression of p21 was examined on HeLa eel/ line, by immunohistochemistry method. 5-fIuorourasil was used as positive control in cytotoxic, apoptotic assay, and p53 expression. The result showed that calanone has cytotoxic activity on HeLa eel/line, with ICw = 22.887 pg/mL, caused cytotoxicity through apoptotic mechanism, increase p53 tumor suppressor gene expression, while the p21
expression test showed a negative result.
Keywords: Calanone, cytotoxic, HeLa eel/lin
