Reaktivitas Antibodi Polyclonal Ssv Terhadap Antigen Homolog Dan Iteterolog = Reactivity Of Polyclonal Antibodies Against Ssv To Their Homologous And Heterologous Antigens
Polyclonal antibodies for Soybean Stunt Virus (SSV) were produced in white rabbit through the following procedures: approximately 100 pg of purified virions emulsified in Complete Freund\u27s Adjuvant (CFA) were injected intramuscularly at first. In the second and third injection, 150 ,ug of purified virions in Incomplete Freund\u27s Adjuvant (IFA) per injection were injected intramuscularly. Finally, about 300 pg of purified virions were injected intravenously as a booster. The injections were done at 2 weeks interval. Antiserum was collected 5 days after the final injection. Antisera was purified by precipitation in saturated ammonium sulfate. Purified antibody was tested for the titer and reactivitiy of antibodies against the homologous and heterologous antigen. The studies were conducted with non-precoated 1-ELISA test.
This research was able to obtain about 25 ml of specific crude antisera for SSV. The concentration of purified polyclonal antibodies was about 9 mg/mi. The titer of polyclonal antibodies was 10.000 in I-ELISA.Witholit absorbtion with sap of healthy plant, the antibodies could not be use to identify the infected and healthy plant samples. In the following test, the absorbed antibody was used Using antibodies to SSV at a dilution of 1: 1000 and 1 : 10.000 against sap extracts sample of healthy and infected plant at a dilution of 1 : 10 by non-precoated 1-ELISA test, indicated that the antibody could be used to identify the healthy and infected samples. By the same test, the antibody could be reacted to both homologous antigen (SSV) and heterologous antigen (CMV isolated from banana).
Key words: SSV, polyclonal antibodies, ELIS
