ABSTRACT
Flow cytometry analysis was successfully used to measure the ploidy of shallot and Japanese bunching onion plants using isolated cell nuclei. In addition, when corrected for ploidy, the nuclear DNA content of shallot cells was found to be twice that of Japanese bunching onion. The chimera phenomenon in tetraploid shallot was also demonstrated.
Key words: flow cytometry, Japanese bunching onion, ploidy, shallot.
INTISARI
Flow coometer berhasil digunakan dalam menganalisa tingkat ploidy tanaman bawang merah dan bawang daun dengan isolasi inti sel. Selain itu berkaitan dengan tingkat ploidy. kandungan DNA inti sel pada tanaman bawang merah didapatkan dua kali lebih besar dsbanding kandungan DNA inti sel pada tanaman bawang daun. Fenomena chimera juga dapat terlihat pada tanaman bawang merah tetraploid.
Kata kunci: flow cytometry, tingkat ploidy, bawang merah, bawang dau
