Determination of intraspecies variations of the V2 region of the 16S rRNA gene of Streptococcus equi subsp. zooepidemicus isolated from pigs, monkeys and humans on the island of Bali Indonesia

Abstract

The 16S rRNA gene of 15 Streptococcus equi subsp. zooepidemicus isolated from pigs, monkeys, and humans was amplified by polymerase chain reaction and subsequently digested with the restriction enzyme HincIl. A restriction profile with two fragments size of 1250 by and 200 by could be observed for all S. equi subsp. zooepidemicus isolated from pigs and monkeys indicating a sequence variable within the V2 region of the 16S rRNA gene of the remaining S. equi subsp. zooepidemicus human isolates. The sequence of the V2 region of S. equi subsp. zooepidemicus isolated from pigs and monkeys revealed an identical (100 per cent similarity) sequence with the V2 region of S. equi subsp. equi ATCC (reference strains). The sequence of the S. equi subsp. zooepidemicus isolated from humans appeared to be similar (88.5 per cent similarity) to the S. equi subsp. equi reference stains. A single amplicon of the spacer region gene encoding the 16S-23S rRNA of 950 by could be observed for S. equi subsp. zooepidemicus isolated from pigs and monkeys, and an amplicon of 650 by for S. equi subsp. zooepidemicus human isolates. Keywords: Streptococcus equi subsp. zooepidemicus â 16S rRNA gene â V2 region â 16-23S rRNA intergenic spacer regio