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羊草LcCBF6基因的表达特性和功能研究
Authors
刘公社
新巴音
+7 more
李倩
李晓霞
杨伟光
程丽琴
陈双燕
高利军
齐冬梅
Publication date
1 January 2021
Publisher
Abstract
羊草是我国重要的牧草和生态草资源,具有耐盐碱、耐旱、耐低温等特性,是天然的抗逆基因资源库。CBF/DREB属于AP2转录因子家族,在植物抗逆中发挥着重要作用。本研究克隆得到羊草LcCBF6(Leymus chinensis Crepeat binding factor 6)基因,该基因含有AP2结构域,编码245个氨基酸。氨基酸序列比对发现,LcCBF6与蒙古冰草和黑麦的CBF6蛋白的同源性分别为92%和91%。组织特异性表达模式分析表明,LcCBF6基因在羊草根、叶、种子中均有表达,且受盐胁迫诱导表达。过表达LcCBF6能显著提高转基因拟南芥的抗盐性。在盐胁迫条件下,转基因株系的绿色子叶数、根长、植株生物量以及存活率等均明显高于野生型。上述结果表明羊草LcCBF6基因在提高植物盐胁迫抗性方面发挥了重要的作用,将为牧草及重要农作物抗逆分子育种提供优异的基因资源
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of Botany,Chinese Academy Of Sciences
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Last time updated on 09/06/2022