The complete genome sequence of Bluetongue virus (BTV) serotype 17 strain 17/BRA/2014/73, isolated from a sheep in Brazil in 2014, is reported here. All segments clustered with western topotype strains and indicated reassortment events with other BTV from the Americas. The strain 17/BRA/2014/73 represents a novel reference strain for BTV-17 from South America

Costa, Érica Azevedo

Driemeier, David

Guedes, Maria Isabel Maldonado Coelho

Guimarães, Lorena Lima Barbosa

Lobato, Zélia Inês Portela

Matos, Ana Carolina Diniz

Mertens, Peter P.C.

Nomikou, Kyriaki

Rosa, Júlio César Câmara

PubMed

Name not available

Genome sequence of Bluetongue virus serotype 17 isolated in Brazil in 2014

Nottingham ePrints

Matos, Ana Carolina Diniz and Rosa, Júlio César Câmara and Nomikou, Kyriaki and Guimarães, Lorena Lima Barbosa and Costa, Érica Azevedo and Guedes, Maria Isabel Maldonado Coelho and Driemeier, David and Lobato, Zélia Inês Portela and Mertens, Peter Paul Clement (2016) Genome sequence of Bluetongue virus serotype 17 isolated in Brazil in 2014. Genome Announcements, 4 (5). e01161/1-e01161/2. ISSN 2169-8287 Access from the University of Nottingham repository: http://eprints.nottingham.ac.uk/38962/1/Genome%20Sequence%20of%20Bluetongue%20virus%20Serotype%2017%20Isolated%20in%20Brazil.pdfCopyright and reuse: The Nottingham ePrints service makes this work by researchers of the University of Nottingham available open access under the following conditions.This article is made available under the Creative Commons Attribution licence and may be reused according to the conditions of the licence.  For more details see: http://creativecommons.org/licenses/by/2.5/A note on versions: The version presented here may differ from the published version or from the version of record. If you wish to cite this item you are advised to consult the publisher’s version. Please see the repository url above for details on accessing the published version and note that access may require a subscription.For more information, please contact eprints@nottingham.ac.ukGenome Sequence of Bluetongue virus Serotype 17 Isolated in Brazilin 2014Ana Carolina Diniz Matos,a,b Júlio César Câmara Rosa,a Kyriaki Nomikou,b Lorena Lima Barbosa Guimarães,c Érica Azevedo Costa,aMaria Isabel Maldonado Coelho Guedes,a David Driemeier,c Zélia Inês Portela Lobato,a Peter Paul Clement Mertensb,dUniversidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazila; Vector-borne Viral Diseases, Arbovirus Molecular Research Group, The Pirbright Institute,Woking, Surrey, United Kingdomb; Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazilc; School of Veterinary Medicine and Science,University of Nottingham, Sutton Bonington Campus, Leicestershire, United KingdomdThe complete genome sequence of Bluetongue virus (BTV) serotype 17 strain 17/BRA/2014/73, isolated from a sheep in Brazil in2014, is reported here. All segments clustered with western topotype strains and indicated reassortment events with other BTVfrom the Americas. The strain 17/BRA/2014/73 represents a novel reference strain for BTV-17 from South America.Received 24 August 2016 Accepted 8 September 2016 Published 27 October 2016CitationMatos ACD, Rosa JCC, Nomikou K, Guimarães LLB, Costa ÉA, Guedes MIMC, Driemeier D, Lobato ZIP, Mertens PPC. 2016. Genome sequence of Bluetongue virusserotype 17 isolated in Brazil in 2014. Genome Announc 4(5):e01161-16. doi:10.1128/genomeA.01161-16.Copyright © 2016 Matos et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.Address correspondence to Zélia Inês Portela Lobato, ziplobato@gmail.com.Bluetongue virus (BTV) is an arbovirus transmitted between itsruminant hosts by biting midges (Culicoides spp.), causingsevere hemorrhagic disease in sheep anddeer (1). BTV contains 10double-stranded RNA (dsRNA) genome segments that encodeseven structural proteins (VP1 to VP7) and five nonstructuralproteins (NS1 to NS4 and S10-ORF2) (2–7). Twenty-seven BTVserotypes have been recognized to date (8, 9).BTV is widespread around theworld (between 40°S and 50°N).Phylogenetic analysis showed that BTV strains evolved separately,with regional variants, named topotypes, for each genome seg-ment. In South America, serological surveys indicated that BTVcirculation is widespread (10). BTV-4 has been isolated in north-ern Argentina and southeastern Brazil (11–14), and BTV-12 wasisolated and associated with BT outbreaks in southern Brazil (15,16). In French Guiana, eight serotypes (BTV-1, -2, -6, -10, -12,-13, -17, and -24) were isolated from ruminants withmild clinicalsigns or even that were asymptomatic (17). Multiple additionalBTV serotypes (BTV-3, -14, -18, -19, and -22) were isolated inBrazil associated with deaths of Brocket deer (Mazama nana) in2015 and 2016 (18). Although there are multiple BTV isolatesfrom South America, complete genome sequences are not avail-able for these isolates, and few phylogenetic analyses have beenpublished (11).In 2014, an outbreak of hemorrhagic disease, with a mortalityrate of 28.89%, affected a flock with 45 Texel sheep in Cachoeirado Sul, Rio Grande do Sul, Brazil (30°02=20S, 52°53=38W). BTVwas isolated from the blood of one sheep after being passagedthree times in the KC cell line and then once in BHK-21 cells. Theisolate was identified as BTV-17 by reverse transcription-PCR(RT-PCR) (8) and designated 17/BRA/2014/73.Genomic dsRNA of the isolate was extracted from infectedBHK-21 cells as the template for the synthesis of full-lengthcDNAs by RT-PCR, and then sequenced on a 3730 ABI capillarysequencer using segment-specific primers (19). Seg-1 to Seg-10 of17/BRA/2014/73 were 3,944, 2,874, 2,768, 1,981, 1,769, 1,638,1,156, 1,125, 1,049, and 822 bp, encoding VP1 (1,302 amino acids[aa]), VP2 (955 aa), VP3 (901 aa), VP4 (644 aa),NS1 (552 aa), VP5(526 aa), VP7 (349 aa), NS2 (354 aa), VP6/NS4 (329/77 aa), andNS3/NS3a/S10-ORF2 (229/216/59 aa), respectively.Phylogenetic analysis of 17/BRA/2014/73 showed 89.2% nu-cleotide (nt) identity in Seg-2/VP2 with the African referencestrain of BTV-17 (RSArrrr/17, accession no. AJ585138), confirm-ing the isolate as the BTV-17western topotype (8). All of the othergenome segments showed greatest similarity to BTV strains fromthe Americas subgroup of the major western lineage/topotype.Seg-1/VP1, Seg-6/VP5, and Seg-10/NS3/NS3a/S10-ORF2 wereclosest (95%, 93.7%, and 99.2% nt identity, respectively) to Ar-gentinian BTV-4 strains from 2001/2002 (strain ARG2002/01,and BTV-4-2001, accession numbers JX024952 and JX024954, re-spectively). Seg-3/VP3, Seg-4/VP4, and Seg-8/NS2 of 17/BRA/2014/73 were closest (98.3%, 98.1%, and 98% nt identity, respec-tively) to BTV-12 isolate BRA2002/01. Seg-5/NS1, Seg-7/VP7,and Seg-9/VP6-NS4 of 17/BRA/2014/73 were closest (97.6%,98.7%, and 98.3% nt identity, respectively) to BTV-2 fromUSA(2003 to 2010; accession numbers KF986502, JQ822254, andKF986513, respectively). These data suggest that 17/BRA/2014/73 has emerged through reassortment of other BTV strainscirculating in the Americas. The strain 17/BRA/2014/73 repre-sents a novel reference strain for BTV-17 from South America.Accession number(s). The genome sequences of BTV-17strain 17/BRA/2014/73 were deposited in GenBank under the ac-cession numbers KX599359 to KX599368.ACKNOWLEDGMENTSWe thank collaborators for providing samples and virus isolates for testsand analysis.This workwas supported byDefra–United Kingdom (project SE1470)and CNPq/CAPES (PVE, grant no. 2392/2013). P.P.C.M. is a Jenner In-vestigator. Z.I.P.L. is a CNPq research fellow.crossmarkGenome AnnouncementsSeptember/October 2016 Volume 4 Issue 5 e01161-16 genomea.asm.org 1FUNDING INFORMATIONThis work, including the efforts of Ana Carolina Diniz Matos,Kyriaki Nomikou, and Peter Mertens, was funded by Department forEnvironment, Food and Rural Affairs (Defra) (SE1470). This work,including the efforts of Ana Carolina Diniz Matos, Júlio César CâmaraRosa, Maria Isabel Maldonado Coelho Guedes, Zélia Inês Portela Lobato,and Peter Mertens, was funded by MCTI | Conselho Nacional deDesenvolvimento Científico e Tecnológico (CNPq) (PVE-185/2012).The funders had no role in study design, data collection and interpreta-tion, or the decision to submit the work for publication.REFERENCES1. Maclachlan NJ, Drew CP, Darpel KE, Worwa G. 2009. The pathologyand pathogenesis of bluetongue. J Comp Pathol 141:1–16. http://dx.doi.org/10.1016/j.jcpa.2009.04.003.2. Huismans H, Els HJ. 1979. Characterization of the tubules associatedwith the replication of three different orbiviruses. Virology 92:397–406.http://dx.doi.org/10.1016/0042-6822(79)90144-2.3. Roy P. 1992. Bluetongue virus proteins. J Gen Virol 73:3051–3064. http://dx.doi.org/10.1099/0022-1317-73-12-3051.4. Firth AE. 2008. Bioinformatic analysis suggests that the orbivirus VP6cistron encodes an overlapping gene. Virol J 5:48. http://dx.doi.org/10.1186/1743-422X-5-48.5. Belhouchet M, Mohd Jaafar F, Firth AE, Grimes JM, Mertens PP,Attoui H. 2011. Detection of a fourth orbivirus non-structural protein.PLoS One 6:e25697. http://dx.doi.org/10.1371/journal.pone.0025697.6. Stewart M, Hardy A, Barry G, Pinto RM, Caporale M, Melzi E, HughesJ, Taggart A, Janowicz A, Varela M, Ratinier M, Palmarini M. 2015.Characterization of a second open reading frame in genome segment 10 ofbluetongue virus. J Gen Virol 96:3280–3293. http://dx.doi.org/10.1099/jgv.0.000267.7. Grimes JM, Burroughs JN, Gouet P, Diprose JM, Malby R, Ziéntara S,Mertens PP, Stuart DI. 1998. The atomic structure of the bluetonguevirus core. Nature 395:470–478. http://dx.doi.org/10.1038/26694.8. Maan NS, Maan S, Belaganahalli MN, Ostlund EN, Johnson DJ, Nomi-kou K, Mertens PP. 2012. Identification and differentiation of the twentysix bluetongue virus serotypes by RT-PCR amplification of the serotype-specific genome segment 2. PLoS One 7:e32601. http://dx.doi.org/10.1371/journal.pone.0032601.9. Jenckel M, Bréard E, Schulz C, Sailleau C, Viarouge C, Hoffmann B,Höper D, Beer M, Zientara S. 2015. Complete coding genome sequenceof putative novel bluetongue virus serotype 27. Genome Announc 3(2):e00016-15. http://dx.doi.org/10.1128/genomeA.00016-15.10. Portela Lobato ZI, Maldonado Coelho Guedes MI, Diniz Matos AC.2015. Bluetongue and other orbiviruses in South America: gaps and chal-lenges. Vet Ital 51:253–262.11. Legisa D, Gonzalez F, De Stefano G, Pereda A, Dus Santos MJ. 2013.Phylogenetic analysis of bluetongue virus serotype 4 field isolates fromArgentina. J Gen Virol 94:652–662.12. Lima PA, Utiumi KU, Yumi K, Nakagaki R, Biihrer DA, AlbuquerqueAS, Rezende FS, Carolina A, Matos D, Inês Z, Lobato P, Driemeier D,Peconick AP, Varaschin MS, Raymundo DL. 2016. Diagnoses of ovineinfection by the serotype-4 bluetongue virus onMinas Gerais, Brazil. ActaSci Vet 44:1–5.13. Balaro MFA, dos Santos Lima M, Del Fava C, de Oliveira GR, PitucoEM, Brandao FZ. 2014. Outbreak of bluetongue virus serotype 4 in dairysheep in Rio de Janeiro, Brazil. J Vet Diagn Invest 26:567–570. http://dx.doi.org/10.1177/1040638714538020.14. Groocock CM, Campbell CH. 1982. Isolation of an exotic serotype ofbluetongue virus from imported cattle in quarantine. Can J Comp Med46:160–164.15. Clavijo A, Sepulveda L, Riva J, Pessoa-Silva M, Tailor-Ruthes A, LopezJW. 2002. Isolation of bluetongue virus serotype 12 from an outbreak ofthe disease in South America. Vet Rec 151:301–302. http://dx.doi.org/10.1136/vr.151.10.301.16. Antoniassi NAB, Pavarini SP, Henzel A, Flores EF, Driemeier D. 2010.Aspiration pneumonia associated with oesophageal myonecrosis in sheepdue to BTV infection in Brazil. Vet Rec 166:52–53. http://dx.doi.org/10.1136/vr.b4775.17. Viarouge C, Lancelot R, Rives G, Bréard E, Miller M, Baudrimont X,Doceul V, Vitour D, Zientara S, Sailleau C. 2014. Identification ofbluetongue virus and epizootic hemorrhagic disease virus serotypes inFrench Guiana in 2011 and 2012. Vet Microbiol 174:78–85. http://dx.doi.org/10.1016/j.vetmic.2014.09.006.18. OIE (World Organisation for Animal Health). 2016. World Animal HealthInformation Database (WAHIS interface). Exceptional epidemiological events,Brazil.WorldOrganisationforAnimalHealth,Paris,France.http://www.oie.int/wahis_2/public/wahid.php/Countryinformation/Countryreports.19. Maan S, Rao S, Maan NS, Anthony SJ, Attoui H, Samuel AR, MertensPP. 2007. Rapid cDNA synthesis and sequencing techniques for the ge-netic study of bluetongue and other dsRNA viruses. J Virol Methods 143:132–139. http://dx.doi.org/10.1016/j.jviromet.2007.02.016.Matos et al.Genome Announcements2 genomea.asm.org September/October 2016 Volume 4 Issue 5 e01161-16

Nottingham eTheses

ABSTRACT
          
            The complete genome sequence of
            Bluetongue virus
            (BTV) serotype 17 strain 17/BRA/2014/73, isolated from a sheep in Brazil in 2014, is reported here. All segments clustered with western topotype strains and indicated reassortment events with other BTV from the Americas. The strain 17/BRA/2014/73 represents a novel reference strain for BTV-17 from South America.
          </jats:p

Ana Carolina Diniz Matos

Júlio César Câmara Rosa

Kyriaki Nomikou

Lorena Lima Barbosa Guimarães

Érica Azevedo Costa

Maria Isabel Maldonado Coelho Guedes

David Driemeier

Zélia Inês Portela Lobato

Peter Paul Clement Mertens

Crossref

Genome Announcements

Genome Sequence of
            <i>Bluetongue virus</i>
            Serotype 17 Isolated in Brazil in 2014

Mertens, Peter Paul Clement

Enlighten

Genome Sequence of Bluetongue virus Serotype 17 Isolated in Brazilin 2014Ana Carolina Diniz Matos,a,b Júlio César Câmara Rosa,a Kyriaki Nomikou,b Lorena Lima Barbosa Guimarães,c Érica Azevedo Costa,aMaria Isabel Maldonado Coelho Guedes,a David Driemeier,c Zélia Inês Portela Lobato,a Peter Paul Clement Mertensb,dUniversidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazila; Vector-borne Viral Diseases, Arbovirus Molecular Research Group, The Pirbright Institute,Woking, Surrey, United Kingdomb; Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazilc; School of Veterinary Medicine and Science,University of Nottingham, Sutton Bonington Campus, Leicestershire, United KingdomdThe complete genome sequence of Bluetongue virus (BTV) serotype 17 strain 17/BRA/2014/73, isolated from a sheep in Brazil in2014, is reported here. All segments clustered with western topotype strains and indicated reassortment events with other BTVfrom the Americas. The strain 17/BRA/2014/73 represents a novel reference strain for BTV-17 from South America.Received 24 August 2016 Accepted 8 September 2016 Published 27 October 2016Citation Matos ACD, Rosa JCC, Nomikou K, Guimarães LLB, Costa ÉA, Guedes MIMC, Driemeier D, Lobato ZIP, Mertens PPC. 2016. Genome sequence of Bluetongue virusserotype 17 isolated in Brazil in 2014. Genome Announc 4(5):e01161-16. doi:10.1128/genomeA.01161-16.Copyright © 2016 Matos et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.Address correspondence to Zélia Inês Portela Lobato, ziplobato@gmail.com.Bluetongue virus (BTV) is an arbovirus transmitted between itsruminant hosts by biting midges (Culicoides spp.), causingsevere hemorrhagic disease in sheep and deer (1). BTV contains 10double-stranded RNA (dsRNA) genome segments that encodeseven structural proteins (VP1 to VP7) and five nonstructuralproteins (NS1 to NS4 and S10-ORF2) (2–7). Twenty-seven BTVserotypes have been recognized to date (8, 9).BTV is widespread around the world (between 40°S and 50°N).Phylogenetic analysis showed that BTV strains evolved separately,with regional variants, named topotypes, for each genome seg-ment. In South America, serological surveys indicated that BTVcirculation is widespread (10). BTV-4 has been isolated in north-ern Argentina and southeastern Brazil (11–14), and BTV-12 wasisolated and associated with BT outbreaks in southern Brazil (15,16). In French Guiana, eight serotypes (BTV-1, -2, -6, -10, -12,-13, -17, and -24) were isolated from ruminants with mild clinicalsigns or even that were asymptomatic (17). Multiple additionalBTV serotypes (BTV-3, -14, -18, -19, and -22) were isolated inBrazil associated with deaths of Brocket deer (Mazama nana) in2015 and 2016 (18). Although there are multiple BTV isolatesfrom South America, complete genome sequences are not avail-able for these isolates, and few phylogenetic analyses have beenpublished (11).In 2014, an outbreak of hemorrhagic disease, with a mortalityrate of 28.89%, affected a flock with 45 Texel sheep in Cachoeirado Sul, Rio Grande do Sul, Brazil (30°02=20S, 52°53=38W). BTVwas isolated from the blood of one sheep after being passagedthree times in the KC cell line and then once in BHK-21 cells. Theisolate was identified as BTV-17 by reverse transcription-PCR(RT-PCR) (8) and designated 17/BRA/2014/73.Genomic dsRNA of the isolate was extracted from infectedBHK-21 cells as the template for the synthesis of full-lengthcDNAs by RT-PCR, and then sequenced on a 3730 ABI capillarysequencer using segment-specific primers (19). Seg-1 to Seg-10 of17/BRA/2014/73 were 3,944, 2,874, 2,768, 1,981, 1,769, 1,638,1,156, 1,125, 1,049, and 822 bp, encoding VP1 (1,302 amino acids[aa]), VP2 (955 aa), VP3 (901 aa), VP4 (644 aa), NS1 (552 aa), VP5(526 aa), VP7 (349 aa), NS2 (354 aa), VP6/NS4 (329/77 aa), andNS3/NS3a/S10-ORF2 (229/216/59 aa), respectively.Phylogenetic analysis of 17/BRA/2014/73 showed 89.2% nu-cleotide (nt) identity in Seg-2/VP2 with the African referencestrain of BTV-17 (RSArrrr/17, accession no. AJ585138), confirm-ing the isolate as the BTV-17 western topotype (8). All of the othergenome segments showed greatest similarity to BTV strains fromthe Americas subgroup of the major western lineage/topotype.Seg-1/VP1, Seg-6/VP5, and Seg-10/NS3/NS3a/S10-ORF2 wereclosest (95%, 93.7%, and 99.2% nt identity, respectively) to Ar-gentinian BTV-4 strains from 2001/2002 (strain ARG2002/01,and BTV-4-2001, accession numbers JX024952 and JX024954, re-spectively). Seg-3/VP3, Seg-4/VP4, and Seg-8/NS2 of 17/BRA/2014/73 were closest (98.3%, 98.1%, and 98% nt identity, respec-tively) to BTV-12 isolate BRA2002/01. Seg-5/NS1, Seg-7/VP7,and Seg-9/VP6-NS4 of 17/BRA/2014/73 were closest (97.6%,98.7%, and 98.3% nt identity, respectively) to BTV-2 from USA(2003 to 2010; accession numbers KF986502, JQ822254, andKF986513, respectively). These data suggest that 17/BRA/2014/73 has emerged through reassortment of other BTV strainscirculating in the Americas. The strain 17/BRA/2014/73 repre-sents a novel reference strain for BTV-17 from South America.Accession number(s). The genome sequences of BTV-17strain 17/BRA/2014/73 were deposited in GenBank under the ac-cession numbers KX599359 to KX599368.ACKNOWLEDGMENTSWe thank collaborators for providing samples and virus isolates for testsand analysis.This work was supported by Defra–United Kingdom (project SE1470)and CNPq/CAPES (PVE, grant no. 2392/2013). P.P.C.M. is a Jenner In-vestigator. Z.I.P.L. is a CNPq research fellow.crossmarkGenome AnnouncementsSeptember/October 2016 Volume 4 Issue 5 e01161-16 genomea.asm.org 1Downloaded from https://journals.asm.org/journal/genomea on 02 November 2021 by 2.99.84.138.FUNDING INFORMATIONThis work, including the efforts of Ana Carolina Diniz Matos,Kyriaki Nomikou, and Peter Mertens, was funded by Department forEnvironment, Food and Rural Affairs (Defra) (SE1470). This work,including the efforts of Ana Carolina Diniz Matos, Júlio César CâmaraRosa, Maria Isabel Maldonado Coelho Guedes, Zélia Inês Portela Lobato,and Peter Mertens, was funded by MCTI | Conselho Nacional deDesenvolvimento Científico e Tecnológico (CNPq) (PVE-185/2012).The funders had no role in study design, data collection and interpreta-tion, or the decision to submit the work for publication.REFERENCES1. Maclachlan NJ, Drew CP, Darpel KE, Worwa G. 2009. The pathologyand pathogenesis of bluetongue. J Comp Pathol 141:1–16. http://dx.doi.org/10.1016/j.jcpa.2009.04.003.2. Huismans H, Els HJ. 1979. Characterization of the tubules associatedwith the replication of three different orbiviruses. Virology 92:397– 406.http://dx.doi.org/10.1016/0042-6822(79)90144-2.3. Roy P. 1992. Bluetongue virus proteins. J Gen Virol 73:3051–3064. http://dx.doi.org/10.1099/0022-1317-73-12-3051.4. Firth AE. 2008. Bioinformatic analysis suggests that the orbivirus VP6cistron encodes an overlapping gene. Virol J 5:48. http://dx.doi.org/10.1186/1743-422X-5-48.5. Belhouchet M, Mohd Jaafar F, Firth AE, Grimes JM, Mertens PP,Attoui H. 2011. Detection of a fourth orbivirus non-structural protein.PLoS One 6:e25697. http://dx.doi.org/10.1371/journal.pone.0025697.6. Stewart M, Hardy A, Barry G, Pinto RM, Caporale M, Melzi E, HughesJ, Taggart A, Janowicz A, Varela M, Ratinier M, Palmarini M. 2015.Characterization of a second open reading frame in genome segment 10 ofbluetongue virus. J Gen Virol 96:3280 –3293. http://dx.doi.org/10.1099/jgv.0.000267.7. Grimes JM, Burroughs JN, Gouet P, Diprose JM, Malby R, Ziéntara S,Mertens PP, Stuart DI. 1998. The atomic structure of the bluetonguevirus core. Nature 395:470 – 478. http://dx.doi.org/10.1038/26694.8. Maan NS, Maan S, Belaganahalli MN, Ostlund EN, Johnson DJ, Nomi-kou K, Mertens PP. 2012. Identification and differentiation of the twentysix bluetongue virus serotypes by RT-PCR amplification of the serotype-specific genome segment 2. PLoS One 7:e32601. http://dx.doi.org/10.1371/journal.pone.0032601.9. Jenckel M, Bréard E, Schulz C, Sailleau C, Viarouge C, Hoffmann B,Höper D, Beer M, Zientara S. 2015. Complete coding genome sequenceof putative novel bluetongue virus serotype 27. Genome Announc 3(2):e00016-15. http://dx.doi.org/10.1128/genomeA.00016-15.10. Portela Lobato ZI, Maldonado Coelho Guedes MI, Diniz Matos AC.2015. Bluetongue and other orbiviruses in South America: gaps and chal-lenges. Vet Ital 51:253–262.11. Legisa D, Gonzalez F, De Stefano G, Pereda A, Dus Santos MJ. 2013.Phylogenetic analysis of bluetongue virus serotype 4 field isolates fromArgentina. J Gen Virol 94:652– 662.12. Lima PA, Utiumi KU, Yumi K, Nakagaki R, Biihrer DA, AlbuquerqueAS, Rezende FS, Carolina A, Matos D, Inês Z, Lobato P, Driemeier D,Peconick AP, Varaschin MS, Raymundo DL. 2016. Diagnoses of ovineinfection by the serotype-4 bluetongue virus on Minas Gerais, Brazil. ActaSci Vet 44:1–5.13. Balaro MFA, dos Santos Lima M, Del Fava C, de Oliveira GR, PitucoEM, Brandao FZ. 2014. Outbreak of bluetongue virus serotype 4 in dairysheep in Rio de Janeiro, Brazil. J Vet Diagn Invest 26:567–570. http://dx.doi.org/10.1177/1040638714538020.14. Groocock CM, Campbell CH. 1982. Isolation of an exotic serotype ofbluetongue virus from imported cattle in quarantine. Can J Comp Med46:160 –164.15. Clavijo A, Sepulveda L, Riva J, Pessoa-Silva M, Tailor-Ruthes A, LopezJW. 2002. Isolation of bluetongue virus serotype 12 from an outbreak ofthe disease in South America. Vet Rec 151:301–302. http://dx.doi.org/10.1136/vr.151.10.301.16. Antoniassi NAB, Pavarini SP, Henzel A, Flores EF, Driemeier D. 2010.Aspiration pneumonia associated with oesophageal myonecrosis in sheepdue to BTV infection in Brazil. Vet Rec 166:52–53. http://dx.doi.org/10.1136/vr.b4775.17. Viarouge C, Lancelot R, Rives G, Bréard E, Miller M, Baudrimont X,Doceul V, Vitour D, Zientara S, Sailleau C. 2014. Identification ofbluetongue virus and epizootic hemorrhagic disease virus serotypes inFrench Guiana in 2011 and 2012. Vet Microbiol 174:78 – 85. http://dx.doi.org/10.1016/j.vetmic.2014.09.006.18. OIE (World Organisation for Animal Health). 2016. World Animal HealthInformation Database (WAHIS interface). Exceptional epidemiological events,Brazil.WorldOrganisationforAnimalHealth,Paris,France.http://www.oie.int/wahis_2/public/wahid.php/Countryinformation/Countryreports.19. Maan S, Rao S, Maan NS, Anthony SJ, Attoui H, Samuel AR, MertensPP. 2007. Rapid cDNA synthesis and sequencing techniques for the ge-netic study of bluetongue and other dsRNA viruses. J Virol Methods 143:132–139. http://dx.doi.org/10.1016/j.jviromet.2007.02.016.Matos et al.Genome Announcements2 genomea.asm.org September/October 2016 Volume 4 Issue 5 e01161-16Downloaded from https://journals.asm.org/journal/genomea on 02 November 2021 by 2.99.84.138.

http://dx.doi.org/10.1128/genomeA.01161-16

Genome sequence of Bluetongue virus serotype 17 isolated in Brazil in 2014

Abstract

Similar works

Full text

Available Versions

Name not available

Nottingham ePrints

Nottingham eTheses

Name not available

Crossref

Enlighten