1227-1231Oil spills can have devastating environmental, economical, and social impacts. The use of bioremediation is a preferred technique for cleaning up oil spills because it facilitates the process of naturally converting oil into non-toxic byproducts without any further local environmental disruption. An experimental study was conducted to degrade marine oil spill using beeswax as a biostimulant for bioremediation and also to study the chemical variation. Beeswax, a nutrient source for proliferation of olephic (oil eating) bacteria, was added in intervals of five days and the corresponding dissolved oxygen (DO) was measured using Azide Winkler’s method. The recuperation of the DO concentration from 3.1 mg/l to 7.4 mg/l is an indication of the reduction in oil content. An oxygen sag curve and a graph showing temporal variation of chemical parameters such as nitrite, nitrate, and ammonium were plotted. During the period of study, the oil content reduced from 10 ml to 5 ml. As the addition of biostimulant resulted in an increase in the rate of bioremediation, the use of beeswax as a biostimulant appears to be an effective tool for the clean-up of oil-contaminated marine waters without adverse environmental impact
