256-262Trikatu is a well known polyherbal powder form formulation in Ayurveda consisting of Piper longum L. fruit, Piper nigrum L. fruit and Zingiber officinale Rosc. rhizome in equal amounts, i.e., 1:1:1 ratio. It is prescribed for cold, fever, asthma, cough, respiratory problems and treatment of digestive disorders. The aim of the present study was to develop and validate a densitometric method for the identification and quantification of piperine and 6-gingerol in the crude drug Trikatu as the markers for quality of raw materials and to develop a well characterized formulation of Trikatu as a chewable tablet