<span style="font-size:11.0pt;font-family: "Times New Roman";mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language: HI" lang="EN-GB">Evaluation of the anticancer potential of coffee<span style="font-size:11.0pt;font-family:"Times New Roman";mso-fareast-font-family: "Times New Roman";mso-bidi-font-family:Mangal;mso-ansi-language:EN-GB; mso-fareast-language:EN-IN;mso-bidi-language:HI" lang="EN-GB"> <span style="font-size:11.0pt;font-family:"Times New Roman";mso-fareast-font-family: "Times New Roman";mso-bidi-font-family:Mangal;mso-ansi-language:EN-GB; mso-fareast-language:EN-US;mso-bidi-language:HI" lang="EN-GB">beans: An <i style="mso-bidi-font-style: normal">in vitro</i> study</span></span></span>

Abstract

266-271The aim of the present study was to evaluate the anticancer activity of the ethanol extract of raw coffee beans and identification of its active component. It was found that coffee bean ethanol extract, at concentration of 0.1µg/ml, has potent anti-proliferative effect against HeLa and PA-1 cell lines with decrease in percentage viability to less than 30% after 72 hrs of incubation. Partially purified green TLC fraction was also found to be cytotoxic to HeLa and PA-1 cell lines with reduction in percentage viability to 26.8 % and 13.6%, respectively, after 72 hrs of incubation. The extract and the fraction were able to induce apoptosis and nuclear fragmentation in the cells as evidenced by DNA fragmentation assay and fluorescence microscopy. Flow cytometry analyses confirmed the ability of the green fraction to arrest the cells at G0/G1- phase. Its non-cytotoxicity to human peripheral lymphocytes indicates its safety towards humans