Simultaneous production of ligninolytic enzymes by a temperature and pH tolerant strain of <i style="mso-bidi-font-style:normal">Aspergillus niger</i> under different cultural conditions
81-86Ligninolytic activity, represented by
laccase, lignin peroxidase (LiP) and manganese peroxidase (MnP), of a
temperature and pH tolerant strain of Aspergillus
niger isolated from a temperate location in the Indian Himalayan Region
(IHR), has been studied under different cultural (physico-chemical &
nutritional) conditions. In plate assays, the fungus exhibited ligninolytic
activity at wide range of temperature (5-45°C) and pH (3.5-9.5). In
quantitative estimations, carried out at 15, 25 and 35°C, production of laccase
was favoured by low temperature (15°), while production of LiP and MnP were
favoured by higher temperatures (25 & 35°C). At optimum growth temperature
(25°C), laccase production was the maximum at 7.5 pH. LiP and MnP production
was favoured between 7.5 to 9.5, and 5.5 to 9.5 pH, respectively. Amongst
nutritional sources, nitrogen sources were recorded as better enhancers for
enzyme production, followed by vitamins and carbon sources. Folic acid (0.01%)
was also found to be a good enhancer for production of all the three enzymes