Spray-drying of xylanase from thermophilic fungus <i style="mso-bidi-font-style:normal">Melanocarpus albomyces</i>–Effect of carriers and binders on enzyme stability
89-95A spray-dried
powder formulation obtained from the fermentation broth of the fungus has been
developed. The effect of different additives (carriers and binders) on thermal
deactivation of xylanase has been evaluated at 60°C. Addition of carriers and
binders improves half-life by 6.6-7.0 folds, whereas the synergistic effect of
carrier and binder enhances the half-life by 27.5 folds. Under the optimized
conditions of the spray-drying process, retention of 99.4% specific activity is
obtained with the maximum powder recovery of 62.4% at 140/65.2°C inlet/outlet temperature. The final
spray dried powder produced is found to be brownish, free flowing and
non-hygroscopic. The shelf-life of spray-dried powder is 202 days at 30°C,
making it industrially useful product.
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