868-875Bacillus sp (PE-11), the most
potent producer of alkaline protease was used for the production of alkaline protease.
Response surface
methodology was used to achieve the optimization of the experimental conditions
for the optimal production of alkaline protease. To study the
proposed second-order polynomial model, the central composite experimental design
with multiple linear regression was used to estimate the model coefficients
of the five selected factors. These factors were considered to influence the optimization
process. The best yields were obtained at pH of 8.0, 35oC, rpm 280,
incubation time 72 h, and 7.5 per cent level of salt solution. This methodology
was found to be very efficient and only 36 experiments were needed to assess these
conditions. The model adequacy was very satisfactory, as the
coefficient of determination was 0.93057