554-558Damage induction to tumour target cells (P815)
by direct electric current (DC) was investigated. A 6 min treatment of P815 cells
with DC generated decreased levels of cell viability and proliferation. The
ultrastructural analysis of DC-treated cells revealed the presence of blebs, loss
of cell surface filopodia, and ruptures in cell membrane. Mitochondrial alterations,
swelling of cells, cytoplasmic matrix rarefaction, and cellular debri formation
were also observed. The study shows that tumoural target cells can be damaged by
direct electric current and this approach may provide means to understand the mechanism
of tumour regression induced by electrochemical therapy