399-401Cotyledonary nodes taken alongwith shoot apex
from seedlings of cotton (G. hirsutum) proliferated into shoots
on nutrient agar medium supplemented with cytokinins. In the presence of optimal
plant growth regulators, low light intensity enhanced the number of shoots initiated
per explant in cotton. An average of 33.5±2.9 shoots were obtained from a single
explant cultured for 8 weeks which is about four fold higher than the values reported
in earlier protocols. The isolated shoots were rooted on nutrient agar medium supplemented
with α-naphthalene acetic acid and transferred to soil after acclimatization. Regenerated
plants were morphologically identical to the seed-germinated plants and were fertile