Pathogenic effects of <i>Salmonella enterica </i>subspecies <i>enterica </i>serovar Typhimurium on sprouting and growth of maize

Abstract

1100-1106The study was undertaken to understand effects and survival of S. enterica subspecies enterica serovar Typhimurium (S. Typhimurium), a zoonotic serovar, on maize seed germination and plant growth. All the four strains of S. enteric subspecies enterica serovar Typhimurium significantly reduced germination of maize seeds in sprouting plates as well as in soil. About ≥2.7×103 Salmonella cfu ml-1 of soaking water, while ≥2.7×107 Salmonella cfu g-1 soil were required to significantly inhibit germination of maize. Similar inhibition of germination could be observed using ≥16 mg of bacteria free Salmonella cell lysate (CL) protein per g of soil or ≥0.5 mg of CL protein per ml of soaking water in sprouting plates. At the constant dose of 3.6×107 to 3.8×107 Salmonella cfu or 5 mg cell lysate protein ml-1 of soaking water, four strains of Salmonella significantly reduced germination, however difference between strains was insignificant. After germination too, maize growth was affected both by Salmonella organism and CL with little strain-to-strain variation. All Salmonella persisted in growing plants from 15 to 35 days of plant age and up to 190 days in soil. Maize plants once grown for a week in sterile soil were resistant to invasion of S. enterica subspecies enterica serovar Typhimurium in their leaves even in doses as high as 7.6×109 cfu g-1 of soil. Salmonella persisted better and longer in plants grown from contaminated seed sown in loam soil, but rarely in plants grew in sandy soil. All maize plants had Salmonella in their stumps even after 35 days of sowing irrespective of kind of soil, primary source of infection (soil or seed) and type of S. enterica subspecies enterica serovar Typhimurium strain. The study revealed that Salmonella is not only zoonotic but a phytopathogen also