Early and transient induction of nitric oxide (NO) in infectious bursal disease virus infection is T-cell dependent: A study in cyclosporin-A treated chicken-model
192-196The level of nitric oxide (NO) in the
supernatants of mitogen (PHA) stimulated lymphocyte cultures from infectious bursal
disease (IBD) virus infected T-cell suppressed and immune competent chickens
was monitored. The immune competent chickens when infected with IBD virus
showed 4-6 folds increased levels of NO as compared to uninfected chickens. The
levels of NO in T-cell suppressed chickens were comparable to uninfected
control chickens, in spite of markedly increased hemorrhage suggesting that the
muscular hemorrhage observed in IBD in not solely and directly related with NO
production. The immune suppressed chickens that did not induce NO production
after IBD virus infection showed
more severe lesions and supported
enhanced virus replication. Taken together it may be suggested that NO
production after IBD virus infection, may exert antiviral effect since the
immune-suppressed chickens that failed to induce NO showed more severe disease
and higher magnitude of virus replication, but does not seem to correlate with
the hemorrhagic lesions which in fact may be as a result of the net outcome of
various host-factors and the determinants responsible for virus virulence and virus
clearance