867-872Internally Transcribed Spacers (ITS)
characterization and distribution of Repetitive Extragenic Palindromic (REP) sequences
were studied in the genome of 223 field isolates of Bacillus thuringiensis from
Madurai, India. They were characterized by morphological,
biochemical and molecular methods. One hundred and twenty four of a total 223
isolates
fitted ITS characterization of B. thuringiensis
varieties known. Significant genomic variation was observed among seven isolates
using REP primers. The ITS PCR product (EMBL accession number AJ639659) exhibited
98% nucleotide sequence homology with B. thuringiensis and placed the origin
of indigenous isolate LDC-7 closer to B. thuringiensis on the basis of phylogenetic
analysis