Simultaneous determination of guanosine and 8-hydroxy-guanosine by differential pulse voltammetry

Abstract

1222-1226Differential pulse voltammetry has been used for the simultaneous determination of guanosine and 8-hydroxyguanosine in phosphate buffers. Well-separated differential pulse voltammetric peaks for guanosine and 8-hydroxyguanosine are observed at pyrolytic graphite electrode. The proposed method is simple, rapid and provides a wide determination range of 0.5 μM to 0.1 mM with good reproducibility. Interference of ascorbic acid and some guanine related compounds has also been studied and it is found that they do not interfere at very low concentration level