Optimization of xylanase production from <i style="mso-bidi-font-style:normal">Melanocarpus albomyces</i> using wheat straw extract and its scale up in stirred tank bioreactor
282-289Production of xylanase from agro-industrial
by-product such as wheat straw has been optimized. The wheat straw is subjected
to heat treatment under mild alkaline conditions for the extraction of
xylo-oligosaccarides, which serve as both an inducer and a carbon source for
fungal growth and xylanase production under submerged fermentation. The
statistical approach of response surface methodology has been employed for the
optimization of different nitrogen sources. The production of xylanase is
influenced by the synergic effect of nitrogen sources in the fermentation
medium. Further optimization of other factors such as inoculum age, inoculum
size, initial pH, growth temperature
and agitation significantly enhance xylanase activity to about 425 ± 5 IU/mL in
shake flask using optimized fermentation medium and conditions. The scale up of
fermentation process up to 14 L bioreactor yields a maximum xylanase activity
of about 520 IU/mL in 36 h of fermentation which is 1.22-fold higher than that
obtained at shake flask level