Purification and characterization of lipoxygenase from aromatic and non-aromatic rice (<i>Oryza sativa </i>L.)

Abstract

401-405Lipoxygenase (Lox) has been ex tensively purified from aromatic (Bas-370) and non-aromatic. (Pusa-834) rice varieties. Crude isolates of Lox from the aromatic varieties (Bas-370 and PB-1) showed higher specific activity (4-fold) when compared to non-aromatic varieties (Pusa-677 and Pusa-834). The activity was optimum at pH 8:0 in .all four varieties. Anionic PAGE of Lox from three days old seed lings revealed one extra band (Rm 0.48) III aromatic varieties, besides the presence of a major band (Rm 0.28) in all the four varieties. Elution profile of Lox from Bas-370 and Pusa-834 on DEAE-cellulose column showed three distinct peaks (L-1, L-2 and L-3), L-2 being the major fraction In both the varieties. SDSPAGE of purified L-2 from Bas-370 showed a single band of molecular mass ~88 kDa