Isolation and analysis of genomic clones coding for legumin storage proteins and their copy number determination in chickpea, <i>Cicer arietinum </i>L.

Abstract

1117-1122The genes encoding legumin storage proteins of chickpea were identified initially by Southern blot hybridization of restricted total genomic DNA of chickpea with pea legumin cDNA probes (pDUB6 and pDUB8). Since these probes showed strong homology to chickpea DNA, they were used for screening chickpea genomic library to isolate and ascertain the positive clones. Confirmation and detailed analysis of these clones were then done by restriction analysis and Southern hybridization. The gene copy number of legumin was estimated and found to be 10 to 12 in chickpea genome