201-207In the experimental group (shh inhibited group),
there were significant decreases in the expression of Oct4, Nanog, Shh, GATA4,
Brachyury and Goosecoid, while increases were observed for TAT and Pdx1. The
expression of Sox17 did not differ between two control and experimental groups.
In experimental group, the amount of GSC positive cells was somehow lower but
it seems that there was no difference for Sox17. Shh inhibition induces ESCs to
differentiate toward definitive endoderm by committing mesendodermal lineages.
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