<span style="font-size:13.0pt;mso-bidi-font-size: 10.0pt" lang="EN-GB">Indirect organogenesis from nodal explants of <i>Coccinia grandis</i><span style="mso-bidi-font-style:italic"> (L.) Voigt </span></span>

Abstract

352-354The present study reports an efficient protocol for indirect shoot organogenesis and plantlets regeneration of Coccinia grandis (L.) Voigt from nodal explants. The maximum frequency of organogenic callus induction was observed in MS medium supplemented with 0.1 mg/L 1-naphthaleneacetic acid (NAA) and 1.0 mg/L N6-benzyladenine (BA). Multiple shoot induction was achieved from the surface of callus on regeneration medium [MS nutrients supplemented with NAA, BA and various concentrations of kinetin (KIN) 0.1-2.0 mg/L]. The highest shoot multiplication (90%) with the increased numbers of shoots (8.7±1.8) and the shoot mean length (8.8±0.4 cm) was achieved on MS medium with 0.1 mg/L NAA, 1.0 mg/L BA and 0.5 mg/L KIN. The regenerated shoots were rooted in vitro on MS medium containing 0.1 mg/L IBA. Plantlets with well developed shoot and root systems were successfully acclimatized (83.5%) and exhibited normal morphology and growth characteristics. </span