239-242Safety and immunogenicity of Brucella
abortus RB51 vaccine has been evaluated in an organised dairy farm in India.
All the cattle (n=29) vaccinated with strain RB51 ‘responded’ to the vaccine as
demonstrated by iELISA using acetone killed strain RB51 antigen. The percentage
responders at day 35, 60 and 90 post vaccination were 100%, 95% and 20%,
respectively. Strain RB51 was able to elicit a good IFN- response from vaccinated
animals. The post-vaccination time point analysis indicated that the cumulative
IFN- response of whole blood from vaccinates stimulated with heat killed RB51
antigen was elicited in 80% of calves at
60 days post vaccination. Absence of strain RB51 in the secretions and
excretion and lack of local or systemic reaction
indicated the safety of the vaccine