282-289Oleaginous microbial strains were cultivated
to identify the best oil-producing strain amongst Yarrowia lipolytica (CGMCC
2.1398), Lipomyces starkeyi (CGMCC 2.1608), Rhodosporidium toruloides
(CGMCC 2.1389), Mortierella isabellina (CGMCC 3.3410), Cunninghamella
blakeleana (CGMCC 3.970), and Mycobacterium QJ311. A method for
rapid determination of oil content and fatty acid composition was established
to identify the optimum oil-producing strains. This method had a relative standard
deviation of 4.09%, an average recovery ratio of 97.09% and a detection limit of 0.1β1.0 g. Mortierella
isabellina CGMCC 3.3410 was identified as the best oil-producing strain amongst the six strains tested, with
a total biomass of 75 g/10 L and a lipid content of 35%. A rapid screening
method of oleaginous microorganisms is discussed for the first time