PCR-RFLP and sequence analysis of 12S and 16S rRNA mitochondrial genes of Grey mullets from East coast of India

Abstract

529-534Present study consists PCR-RFLP and sequence analysis of mitochondrial genes (12S and 16S rRNA) on four species of Mugilidae, collected from east coast of India. Three and four restriction enzymes were found informative for 12S rRNA mitochondrial gene for Liza macrolepis and Liza tade respectively. Whereas, eight, seven and eight restriction enzymes were found informative for Liza macrolepis, Liza parsia and Liza tade respectively for 16S rRNA mitochondrial gene. Sequence analysis of 12S rRNA and 16S rRNA revealed that Liza macroplepis was distant from the other two Liza species, L. parsia and L. tade. Mugil cephalus was found to be the most distinct amongst the mullet species analyzed based on sequence analysis of 16S rRNA mitochondrial gene