429-437In the present paper, complementary
DNA-amplified fragment length polymorphism (cDNA-AFLP) was used to examine and
identify differentially expressed genes in Capsicum
annuum exposed to UV-B irradiation. Around 4000 transcript derived
fragments (TDFs) were visualized and in total 183 TDFs were isolated, sequenced
and analyzed by Blast 2 go. Among these TDFs, 84 of them showed homology to
known genes. There were 43 TDFs showing up-regulated
expression, 24 TDFs showing down-regulated expression and 27 TDFs showing
both up-regulated and down-regulated expression, respectively. Some of these
TDFs were found to be in response/related to UV-B stress, including carbonic
anhydrase, calcium-dependent protein, thionin-like protein, bzip protein and so
on. In particular, chlorophyll a/b binding protein (Capcab) responding to UV-B stress was cloned. It was concluded that
Capcab could play a protective role
in plant anti-UV-B and maintaining photosynthetic rate under UV-B stress