Siderophore production by <i style="">Alcaligenes faecalis</i> and its application for growth promotion in <i style="">Arachis hypogaea</i>

Abstract

302-307A rhizobacterium isolated from groundnut rhizosphere and identified as Alcaligenes faecalis BCCM 2374 produced siderophore during 24 h submerged growth in modified succinic acid medium (SM) prepared in tap water without deferration. For the optimized production of siderophore in the presence of starch and sucrose, the optimum parameters found were as follows: slight acidic pH (5.5), 35oC temperature and 1% inoculum. While alkaline pH (8.5), 37oC temperature, 5% inoculum in the presence of fructose, xylose and glucose gave optimum growth, succinic and citric acids, NH4SO4, NH4NO3 and NH4Cl supported growth as well as siderophoregenesis. Amino acids also showed varying effects on growth and siderophore production. Inoculation of A. faecalis enhanced seed germination (8.75%), root length (9.35%), shoot length (16%) and chlorophyll content (8.0%) in Arachis hypogaea over control treatment under pot culture conditions