RAPD analysis in mungbean [<i style="">Vigna radiata</i> (L.) Wilczek.] II: A comparison of efficiency parameters of RAPD primers

Abstract

276-282 The study was undertaken to identify the efficient RAPD primers that could differentiate a set of 39 mungbean [Vigna radiata (L.) Wilczek] genotypes. Various efficiency parameters, namely, discriminatory power (D), discriminatory power with infinite sample size (DL), diversity index (DI), resolving power (Rp) and marker index (MI) were studied for 30 primers. The relationship between the parameters was studied using Spearman rank correlation coefficient. The parameters D and DL were the most efficient, followed by Rp, MI and DI. DI was not a good parameter in those cases where a set of primers with intermediate range of variation in their efficiency were used. None of the 30 primers alone could differentiate all 39 mungbean genotypes. However, a methodology could be designed to find the primer/primers combination to differentiate all 39 genotypes. The primers S-1 and S-2 were more efficient with iso-frequency distribution of most of their banding patterns and a combination of any one of the primers with S-1 or S-2 could identify all the genotypes. The study showed how to select the minimum number of efficient primers aimed to distinguish all genotypes cost-effectively. </smarttagtype