816-819Cellulose is one of the most abundant non-degradable
organic compound on earth. Near about half of the municipal and agricultural
solid wastes contain cellulose or their derivatives. Saccharification or
enzymatic hydrolysis of cellulosic wastes liberates glucose. In the present
work, cellulase from Cellulomonas uda was extracted, partially purified by
dialysis and immobilized on an organic support namely tri(4-formyl phenoxy)
cyanurate. Percentage saccharification of seven different cellulosic waste
materials was studied using native and immobilized cellulase systems. Maximum
saccharification for both native and immobilized cellulase was observed for
sawmill dust or wood dust (4.9 and 2.4% respectively) as compared to other
cellulosic waste substrates