430-432Production of
pharmaceutically useful plant secondary metabolites in vitro has various
advantages compared to extraction of these compounds from plants grown in the
field or collected in nature. Exact control of various environmental parameters
ensures a reproducible quality of the material totally independent from climate,
weather and pests that affect severely the quality of plant material grown in
nature. Thus, much research has been done to establish plant cell and suspension
cultures for metabolite production. However, undifferentiated cell cultures
often do not produce the desired metabolites in considerable amounts or lose
their production capacity over a period of time. In contrast, in vitro
cultures of fully differentiated plant organs exhibit a high and reliable
production capacity of plant secondary metabolites. Thus, special bioreactors
working according to the temporary immersion principle have been designed for
automated in vitro culture of fully differentiated plant organs. It has
been demonstrated that shoots, roots as well as tubers can be grown successfully
with high multiplication rates in these bioreactors. Moreover, it has been found
that metabolite concentrations in these tissues are much higher compared to
undifferentiated cell cultures. Control of in vitro environmental
parameters such as medium supplements, light conditions, immersion frequencies
and gas composition have been used successfully to modify the metabolite content
of the produced plant biomass. This is a very promising strategy for production
of pharmaceutically active plant biomass in vitro