Structural characterization of the <i style="">O</i>-antigenic polysaccharide from the lipopolysaccharide of <i style="">Vibrio</i> <i style="">cholerae</i> <i style="">O</i>37

Abstract

729-734 The chemical structure of the O-antigenic polysaccharide isolated from the lipopolysaccharide of Vibrio cholerae O37 by mild acid hydrolysis was elucidated. The O-antigenic polysac­charide is found to consist of D-glucose, N-acetyl-D-Quinovos­amine and small amount of 4-O-methyl-N-acetyl-D-quinovas­amine. The structure of the O-antigen is established by using sugar and methylation analyses, Smith degradation studies and by using GLC, GC-MS, FAB-MS, one dimensional 1H and 13C NMR spectroscopy and two dimensional NMR spectroscopy including COSY, TOCSY, HSQC, experiments. </smarttagtype