Changes in some biochemical parameters in the liver and muscle of Colisa fasciatus due to toxicity of ethanolic extract of Nerium indicum Mill. (Lal Kaner) latex

Abstract

48-54Present study deals with piscicidal, toxicological and biochemical effects of ethanolic extract of Nerium indicum Mill. (Lal Kaner) latex against freshwater weed fish Colisa fasciatus. There was a significant (P<0.05) negative correlation between LC values and exposure periods i.e. LC50 values decreased from 14.05mg/l (24h) to 5.52mg/l (96h). Sub-lethal exposure of ethanolic latex extract for 24h and 96h caused significant (P<0.05) time and dose dependent alterations in the levels of total protein, total free amino acid, nucleic acid, glycogen, pyruvate, lactate and also in the activity of enzyme protease, alanine aminotransferase, aspartate aminotransferase, acetylcholinesterase, lactic dehydrogenase, succinic dehydrogenase and cytochrome oxidase in liver and muscle tissues of fish. Withdrawal experiments shows, their biochemical effects are reversible in action. Thus, N. indicum latex extract mainly suppress energy production and shifts fish respiration towards the anaerobic segment