The essential oils from the stem barks and leaves of Croton urucurana Baillon (Euphorbiaceae) were analyzed by GC-FID and GC-MS. 100 compounds were identified and borneol (14.7%), bornyl acetate (5.2%), cadina-4,10(14)-dien-1 alpha-ol (14.7%), sesquicineole (10.5%) and gamma-gurjunene epoxide (5.4%) were the main components in essential oil from the steam barks. The oil from leaves was rich in sesquiterpenoids, mainly germacrene-D (15.2%) and bicyclogermacrene (36.4%). The in vitro cytotoxicity of the oils was evaluated against nine cancer cell lines and herbicidal effects against two seeds. The antimicrobial and antioxidant activities of the oils also were evaluated. The essential oil of stem bark was found to be more active than oil from leaves.123250261Fundacao de Apoio ao Desenvolvimento de EnsinoCiencia e Tecnologia de Mato Grosso do Sul (FUNDECT/MS)UFM