'Revista Brasileira de Hematologia e Hemoterapia (RBHH)'
Abstract
As a contribution to the identification and characterization of oral Streptococcus, culture samples of the mutans group, biochemically identified as S. mutans, S. sobrinus and not identified were analysed through the polyacrylamide gel electrophoresis technique, using distinct methods to isolate intracellular proteins. The results showed that the direct resuspension of the whole cell protein in buffered solubilizing solution allowed to distinguish between species to S. mutans and S. sobrinus. The tests performed with resuspended samples in sucrose showed that the osmotic shock procedure gives important data in the characterization of microbial species, but limited by a larger manipulation of the samples. The cell mechanic desintegration method revealed the highest number of bands in the gel and a better resolution of the electrophoretic profiles. In general, the polyacrylamide gel electrophoresis, showed to be a valuable complementary technique to detect differences or to establish similarities among microrganism groups.37225726
