Improving The Differential Diagnosis Between Myelodysplastic Syndromes And Reactive Peripheral Cytopenias By Multiparametric Flow Cytometry: The Role Of B-cell Precursors
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Background: Immunophenotyping is a valuable ancillary technique for the differential diagnosis between myelodysplastic syndromes (MDS) with low bone marrow (BM) blast counts and a normal karyotype, and reactive peripheral (PB) cytopenias. Our aim was to search for the most important variables for this purpose. We also analyzed the age variation of BM B-cell precursors (BCP) and its differences in reactive and clonal cytopenias. Methods: Immunophenotypic analyzes were performed in BM of 54 patients with MDS (76% with BM blasts 55 years. % BM BCP could be calculated by the formula: (-7.97 x log age) + (4.24 x log % CD34(+) cells) -(0.22 x nr. alterations CD34(+) cells) + 0.577. Corrected R-2 = 0.467. Conclusion: Analysis of myelomonocytic precursors and CD34(+) cells was satisfactory for the differential diagnosis between reactive PB cytopenias and MDS. The most specific alterations were found in CD34(+) cells. Comparison of the values obtained with those of normal age-matched controls is recommended.10Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)FAEPEX (Research fund of the University of Campinas) [proc 1208/11]MDS FoundationFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq
