Bacillus safensis is a microorganism recognized for its biotechnological and industrial potential due to its interesting enzymatic portfolio. Here, as a means of gathering information about the importance of this species in oil biodegradation, we report a draft genome sequence of a strain isolated from petroleum.

Angolini, Célio F F

Fonseca, Francine S A

Laborda, Prianda R

Marsaioli, Anita J

Oliveira, Valéria M

Souza, Anete P

Genome Announcements

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Repositorio da Producao Cientifica e Intelectual da Unicamp

Genome Sequence of Bacillus safensis CFA06, Isolated from
Biodegraded Petroleum in Brazil
Prianda R. Laborda,a Francine S. A. Fonseca,b Célio F. F. Angolini,b Valéria M. Oliveira,c Anete P. Souza,a Anita J. Marsaiolib
Centro de Biologia Molecular e Engenharia Genética (CBMEG), Universidade Estadual de Campinas (Unicamp), Campinas, São Paulo, Brazila; Instituto de Química,
Universidade Estadual de Campinas (Unicamp), Campinas, São Paulo, Brazilb; Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas (CPQBA), Universidade
Estadual de Campinas (Unicamp), Campinas, São Paulo, Brazilc
Bacillus safensis is a microorganism recognized for its biotechnological and industrial potential due to its interesting enzymatic
portfolio. Here, as a means of gathering information about the importance of this species in oil biodegradation, we report a draft
genome sequence of a strain isolated from petroleum.
Received 10 June 2014 Accepted 3 July 2014 Published 24 July 2014
Citation Laborda PR, Fonseca FSA, Angolini CFF, Oliveira VM, Souza AP, Marsaioli AJ. 2014. Genome sequence of Bacillus safensis CFA06, isolated from biodegraded petroleum
in Brazil. Genome Announc. 2(4):e00642-14. doi:10.1128/genomeA.00642-14.
Copyright © 2014 Laborda et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license.
Address correspondence to Anita J. Marsaioli, anita@iqm.unicamp.br.
Bacillus safensis is a Gram-positive rod-shaped free-livingspore-forming mesophilic bacterium originally isolated from
a National Aeronautics and Space Administration (NASA) space-
craft assembly facility (1). The presence of the species has also been
reported in desert soil (2), sweet meat whey (3), root tubers (4),
and rhizosphere (5). The search for potentially biodegrading mi-
croorganisms revealed that this species can also be found in petro-
leum samples. Since then, ongoing studies are demonstrating its
environmental relevance in biocatalysis and bioremediation, and
they point to the importance of a genome sequence to comple-
ment biological and chemical findings. The first B. safensis ge-
nome was announced in 2013 and regarded a rhizosphere sample
from a saline desert in India (5). In this paper, we present the
genome sequence of B. safensis strain CFA06, recovered from bio-
degraded oil samples from the Pintassilgo Field at the Potiguar
Basin, in the state of Rio Grande do Norte, Brazil.
A genomic library for B. safensis strain CFA06 was constructed
according to the recommendations of the TruSeq DNA sample
preparation kit (Illumina, Inc.), beginning with 1 g of sheared
DNA. The shotgun sequencing was conducted using one flowcell
lane (clusterizedwith 10 pM) of aGenomeAnalyzer IIx (Illumina,
Inc.) and paired reads of 2 72 bases. The CLCGenomicsWork-
bench version 4.9 (CLC bio) was used for read quality control
(minimum read Q score, 30; maximum ambiguous nucleotides
allowed, 2; minimum read length, 70 bases) and de novo assembly
into contigs (minimum contig length, 450 bp; k-mer, 41). Further
genomic analyses, such as sequence similarity searching and an-
notation, were conducted by the Integrated Microbial Genome
(IMG) Annotation Pipeline (6).
The whole-genome sequencing of B. safensis CFA06 generated
6.8 Gb of data in 94 million paired reads. More than 91% of the
reads passed quality control. The de novo assembly resulted in 65
contigs, with an average length of 58,020 bp (ranging from 495 to
294,018 bp) and an N50 contig length of 103,586 bp. The estimated
genome size is 3,771,318 bp, with 41.47% GC content. Approxi-
mately 70% of the nucleotide positions had coverage ranging from
1,000 to 2,000. A total of 3,781 protein-coding genes were pre-
dicted, 71% of which had a predicted function. Of the 43 RNA genes
identified, 3 are rRNAsand12are tRNAs.Twenty-ninepercentof the
predicted genes (n 1,124) are connected to KEGG pathways.
Nucleotide sequence accessionnumbers.Thiswhole-genome
shotgun project has been deposited at DDBJ/EMBL/GenBank un-
der the accession no. JNBO00000000. The version described in
this paper is version JNBO01000000. The B. safensis genome se-
quences have also been deposited at the IMG Database under the
project ID Gi23929.
ACKNOWLEDGMENTS
This work was supported by Petróleo Brasileiro S.A. (Petrobras) grant
4600284082 and São Paulo Research Foundation (FAPESP) grants 2008/
52197-4 and 2010/06557-9.
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Genome AnnouncementsJuly/August 2014 Volume 2 Issue 4 e00642-14 genomea.asm.org 1


Genome Sequence Of Bacillus Safensis Cfa06, Isolated From Biodegraded Petroleum In Brazil.

http://dx.doi.org/10.1128/genomeA.00642-14

Genome Sequence Of Bacillus Safensis Cfa06, Isolated From Biodegraded Petroleum In Brazil.

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